ARG65414
anti-HLA A2 antibody [BB7.2] (azide free)
anti-HLA A2 antibody [BB7.2] (azide free) for Flow cytometry,Immunoprecipitation and Human
Immune System antibody
Overview
| Product Description | Azide free Mouse Monoclonal antibody [BB7.2] recognizes HLA A2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP |
| Specificity | The clone BB7.2 recognizes an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands within the human HLA-A2 histocompatibility antigen. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | BB7.2 |
| Isotype | IgG2b |
| Target Name | HLA A2 |
| Immunogen | HLA-A2 solubilised by papain. |
| Conjugation | Un-conjugated |
| Alternate Names | MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain |
|---|---|
| Gene Symbol | HLA-A |
| Gene Full Name | major histocompatibility complex, class I, A |
| Background | HLA-A2 (44 kDa) is the most frequent HLA-A allele in human ethnic populations. HLA-A, together with HLA-B and HLA-C, represent human HLA class I major histocompatibility (MHC) antigens. These intrinsic membrane glycoproteins are expressed on nucleated cells and noncovalently associate with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence._x000D_ |
| Function | Involved in the presentation of foreign antigens to the immune system. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 40 kDa |
| PTM | Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). |
Clone References
Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment.
Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.
Down-modulation of CD8αβ is a fundamental activity of primate lentiviral Nef proteins.
FACS / Human
HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/γc(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells.
FACS /
