ARG63005

anti-HLA Class I antibody [MEM-123]

anti-HLA Class I antibody [MEM-123] for ELISA,Flow cytometry,Immunoprecipitation and Human,Bovine,Primates

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-123] recognizes HLA Class I
Tested Reactivity Hu, Bov, NHuPrm
Tested Application ELISA, FACS, IP
Specificity The clone MEM-123 reacts with all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.
MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.
Host Mouse
Clonality Monoclonal
Clone MEM-123
Isotype IgG3
Target Name HLA Class I
Immunogen COS-7 African green monkey kidney cells
Conjugation Un-conjugated
Alternate Names MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS4 µg/ml
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3105 Human HLA-A

Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain

Gene Symbol HLA-A
Gene Full Name major histocompatibility complex, class I, A
Background HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Function Involved in the presentation of foreign antigens to the immune system. [UniProt]
Research Area Immune System antibody
Calculated MW 40 kDa
PTM Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).