ARG53937
anti-HLA Class I antibody [MEM-123] (PE)
anti-HLA Class I antibody [MEM-123] (PE) for Flow cytometry and Human,Primates,Bovine
Immune System antibody
Overview
Product Description | PE-conjugated Mouse Monoclonal antibody [MEM-123] recognizes HLA Class I |
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Tested Reactivity | Hu, Bov, NHuPrm |
Tested Application | FACS |
Specificity | The clone MEM-123 reacts with all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9. |
Host | Mouse |
Clonality | Monoclonal |
Clone | MEM-123 |
Isotype | IgG3 |
Target Name | HLA Class I |
Immunogen | COS-7 African green monkey kidney cells |
Conjugation | PE |
Alternate Names | MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. |
Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
Preservative | 15 mM Sodium azide |
Stabilizer | 0.2% (w/v) high-grade protease free BSA |
Concentration | 0.1 mg/ml |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain |
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Gene Symbol | HLA-A |
Gene Full Name | major histocompatibility complex, class I, A |
Background | HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. |
Function | Involved in the presentation of foreign antigens to the immune system. [UniProt] |
Research Area | Immune System antibody |
Calculated MW | 40 kDa |
PTM | Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). |