ARG63003
anti-HLA Class I antibody [MEM-147]
anti-HLA Class I antibody [MEM-147] for Flow cytometry,Immunoprecipitation,Western blot and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-147] recognizes HLA Class I |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IP, WB |
| Specificity | The clone MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-147 |
| Isotype | IgG1 |
| Target Name | HLA Class I |
| Immunogen | PHA-activated peripheral blood lymphocytes |
| Conjugation | Un-conjugated |
| Alternate Names | MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under non-reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain |
|---|---|
| Gene Symbol | HLA-A |
| Gene Full Name | major histocompatibility complex, class I, A |
| Background | HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. |
| Function | Involved in the presentation of foreign antigens to the immune system. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 40 kDa |
| PTM | Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). |
Clone References
The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex.
