ARG63003

anti-HLA Class I antibody [MEM-147]

anti-HLA Class I antibody [MEM-147] for Flow cytometry,Immunoprecipitation,Western blot and Human

Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [MEM-147] recognizes HLA Class I

Tested Reactivity Hu
Tested Application FACS, IP, WB
Specificity The clone MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types.
MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Host Mouse
Clonality Monoclonal
Clone MEM-147
Isotype IgG1
Target Name HLA Class I
Immunogen PHA-activated peripheral blood lymphocytes
Conjugation Un-conjugated
Alternate Names MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3105 Human HLA-A

Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain

Gene Symbol HLA-A
Gene Full Name major histocompatibility complex, class I, A
Background HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Function Involved in the presentation of foreign antigens to the immune system. [UniProt]
Research Area Immune System antibody
Calculated MW 40 kDa
PTM Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).

Clone References

The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex.

Tran TM et al.
Immunogenetics.,  (2001)

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