ARG41727
anti-HLA DMB antibody
anti-HLA DMB antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
Product Description | Rabbit Polyclonal antibody recognizes HLA DMB |
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Tested Reactivity | Hu |
Tested Application | IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | HLA DMB |
Antigen Species | Human |
Immunogen | Synthetic peptide corresponding to aa. 81-100 of Human HLA DMB. (NQKDTLMQRLRNGLQNCATH) |
Conjugation | Un-conjugated |
Alternate Names | RING7; D6S221E; HLA class II histocompatibility antigen, DM beta chain; MHC class II antigen DMB; Really interesting new gene 7 protein |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Observed Size | ~ 29 kDa |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
Stabilizer | 5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # P28068 Human HLA class II histocompatibility antigen, DM beta chain |
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Gene Symbol | HLA-DMB |
Gene Full Name | major histocompatibility complex, class II, DM beta |
Background | HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
Function | Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. [UniProt] |
Cellular Localization | Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Localizes to late endocytic compartment. Associates with lysosome membranes. [UniProt] |
Calculated MW | 29 kDa |