ARG63019
anti-HLA E antibody [MEM-E/07]
anti-HLA E antibody [MEM-E/07] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-E/07] recognizes HLA E |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, IP |
| Specificity | The clone MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-E/07 |
| Isotype | IgG1 |
| Target Name | HLA E |
| Immunogen | Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide. |
| Conjugation | Un-conjugated |
| Alternate Names | MHC class I antigen E; QA1; EA2.1; HLA-6.2; EA1.2; MHC; HLA class I histocompatibility antigen, alpha chain E |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | IHC-P: Spleen |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13747 Human HLA class I histocompatibility antigen, alpha chain E |
|---|---|
| Gene Symbol | HLA-E |
| Gene Full Name | major histocompatibility complex, class I, E |
| Background | HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| Function | Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 40 kDa |
Clone References
HLA-E monoclonal antibodies recognize shared peptide sequences on classical HLA class Ia: relevance to human natural HLA antibodies.
ELISA / Human
