ARG10105
anti-HLA G antibody [MEM-G/1]
anti-HLA G antibody [MEM-G/1] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [MEM-G/1] recognizes HLA G |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-Fr, IHC-P, WB |
| Specificity | The clone MEM-G/1 reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-G/1 |
| Isotype | IgG1 |
| Target Name | HLA G |
| Antigen Species | Human |
| Immunogen | Denatured bacterially expressed recombinant human HLA-G heavy chain. |
| Conjugation | Un-conjugated |
| Alternate Names | HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Incubated for 1 hour at RT. Staining technique: Standard ABC technique Pretreatment: Heat retrieval in 0.01M Citrate buffer (4x2 min. In microwave oven) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Human placenta. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G |
|---|---|
| Gene Symbol | HLA-G |
| Gene Full Name | major histocompatibility complex, class I, G |
| Background | HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| Function | Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 38 kDa |
Clone References
Differential expression of β-catenin and Dickkopf-1 in the third trimester placentas from normal and preeclamptic pregnancies: a comparative study.
IHC / Human
