ARG43186

anti-HLTF / SMARCA3 antibody

anti-HLTF / SMARCA3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes HLTF / SMARCA3
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HLTF / SMARCA3
Antigen Species Human
Immunogen Synthetic peptide derived from Human HLTF / SMARCA3.
Conjugation Un-conjugated
Alternate Names SNF2L3; RNF80; DNA-binding protein/plasminogen activator inhibitor 1 regulator; EC 6.3.2.-; ZBU1; EC 3.6.4.-; SMARCA3; HLTF1; HIP116A; Sucrose nonfermenting protein 2-like 3; RING finger protein 80; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; HIP116; Helicase-like transcription factor

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562
Observed Size ~ 120 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20585 Mouse HLTF

GeneID: 6596 Human HLTF

Swiss-port # Q14527 Human Helicase-like transcription factor

Swiss-port # Q6PCN7 Mouse Helicase-like transcription factor

Gene Symbol HLTF
Gene Full Name helicase-like transcription factor
Background This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]
Function Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Nuclear localization is stimulated by progesterone. [UniProt]
Calculated MW 114 kDa