ARG66440
anti-HMGN1 phospho (Ser21) antibody
anti-HMGN1 phospho (Ser21) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HMGN1 phospho (Ser21) |
|---|---|
| Tested Reactivity | Hu, Mk |
| Tested Application | ICC/IF, IHC-P |
| Specificity | The antibody detects endogenous levels of HMG-14 protein only when phosphorylated at S21. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HMGN1 |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Ser21 of Human HMGN1. |
| Conjugation | Un-conjugated |
| Alternate Names | HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: High-pressure and temperature EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05114 Human Non-histone chromosomal protein HMG-14 |
|---|---|
| Gene Symbol | HMGN1 |
| Gene Full Name | high mobility group nucleosome binding domain 1 |
| Background | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011] |
| Function | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity). [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus. [UniProt] |
| Calculated MW | 11 kDa |
| PTM | Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization. [UniProt] |
