ARG43898
anti-HPRT1 antibody
anti-HPRT1 antibody for ELISA,Flow cytometry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HPRT1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HPRT1 |
| Antigen Species | Human |
| Immunogen | Human HPRT1 recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | Hypoxanthine-guanine phosphoribosyltransferase |
| Alternate Names | HPRT1; Hypoxanthine Phosphoribosyltransferase 1; HGPRT; HPRT; Hypoxanthine Guanine Phosphoribosyl Transferase; Hypoxanthine-Guanine Phosphoribosyltransferase; EC 2.4.2.8; HGPRTase; Hypoxanthine-Guanine Phosphoribosyltransferase 1; Testicular Tissue Protein Li 89; Lesch-Nyhan Syndrome |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified with Immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P00492 Human Hypoxanthine-guanine phosphoribosyltransferase Swiss-port # P27605 Rat Hypoxanthine-guanine phosphoribosyltransferase |
|---|---|
| Gene Symbol | HPRT1 |
| Gene Full Name | Hypoxanthine Phosphoribosyltransferase 1 |
| Background | The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout. |
| Function | Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. |
| Cellular Localization | Cytoplasm |
| Calculated MW | 25 kDa |
| PTM | Acetylation, Isopeptide bond Phosphoprotein, Ubl conjugation |
Images (4) Click the Picture to Zoom In
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ARG43898 anti-HPRT1 antibody WB image
Western blot: Hela and 293T stained with ARG43898 anti-HPRT1 antibody at 0.5 μg/mL dilution.
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ARG43898 anti-HPRT1 antibody FACS image
Flow Cytometry: 293T cells stained with ARG43898 anti-HPRT1 antibody (blue) at 1 μg/1x10^6 cells dilution.
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ARG43898 anti-HPRT1 antibody WB image
Western blot: Mouse brain stained with ARG43898 anti-HPRT1 antibody at 0.5 μg/mL dilution.
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ARG43898 anti-HPRT1 antibody WB image
Western blot: Rat brain stained with ARG43898 anti-HPRT1 antibody at 0.5 μg/mL dilution.
