ARG65382
anti-Helios antibody [22F6]
anti-Helios antibody [22F6] for Flow cytometry,CyTOF®-candidate and Human,Mouse
Controls and Markers antibody; Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Hamster Monoclonal antibody [22F6] recognizes Helios |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | CyTOF®-candidate, FACS |
| Specificity | The armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymicderived regulatory T cells. |
| Host | Hamster |
| Clonality | Monoclonal |
| Clone | 22F6 |
| Isotype | IgG |
| Target Name | Helios |
| Immunogen | Peptide coresponding to the amino acids 51-107 of Helios |
| Conjugation | Un-conjugated |
| Alternate Names | HELIOS; ZNF1A2; Ikaros family zinc finger protein 2; ANF1A2; Zinc finger protein Helios; ZNFN1A2 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Staining method: - Perform staining of cell surface markers (CD25, CD4 etc.) for 20 min. at RT in the dark; 100 µl of peripheral blood. - Add 3 ml of PBS with 1% BSA, centrifugate at 300g and discard the supernatant. Further steps perform on ice and with ice-cold reagents. - Resuspend the cells in 5 ml of cold fixation solution (Miltenyi Biotec) and incubate for 30 min. on ice. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 5 ml of ice-cold PBS with 1% BSA. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 5 ml of ice-cold permeabilization solution (Miltenyi Biotec) and incubate 5 min. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 80µl of ice-cold permeabilization solution and add 20 µl of FcR blocking solution and incubate for 5 min. (4°C, in the dark). - Perform intracellular staining of Helios for 30 min. (4°C, in the dark) with 10 µl of anti-Helios PE antibody (22F6) in 100 µl. - Add 2 ml of ice-cold permeabilization solution and incubate for 5 min. at 4°C in the dark. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 3 ml of ice-cold PBS with 1% BSA. - Centrifugate for 5 min. at 1000 g, 4°C, and discard the supernatant. - Resuspend the cells in 150 µl of ice-cold PBS with 1% BSA and measure on a FACS device with appropriate setting as soon as possible. Keep cold until measuring. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IKZF2 |
| Gene Full Name | IKAROS family zinc finger 2 (Helios) |
| Background | Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells. |
| Function | Associates with Ikaros at centromeric heterochromatin. [UniProt] |
| Highlight | Related products: Helios antibodies; Anti-Hamster IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Research Area | Controls and Markers antibody; Gene Regulation antibody; Immune System antibody |
| Calculated MW | 60 kDa |
