ARG54195

anti-Helios antibody [22F6] (PE)

anti-Helios antibody [22F6] (PE) for Flow cytometry and Human,Mouse

Controls and Markers antibody; Gene Regulation antibody; Immune System antibody

Overview

Product Description PE-conjugated Hamster Monoclonal antibody [22F6] recognizes Helios
Tested Reactivity Hu, Ms
Tested Application FACS
Specificity The armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymicderived regulatory T cells.
Host Hamster
Clonality Monoclonal
Clone 22F6
Isotype IgG
Target Name Helios
Immunogen Peptide coresponding to the amino acids 51-107 of Helios
Conjugation PE
Alternate Names HELIOS; ZNF1A2; Ikaros family zinc finger protein 2; ANF1A2; Zinc finger protein Helios; ZNFN1A2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22779 Mouse IKZF2

GeneID: 22807 Human IKZF2

Swiss-port # P81183 Mouse Zinc finger protein Helios

Swiss-port # Q9UKS7 Human Zinc finger protein Helios

Gene Symbol IKZF2
Gene Full Name IKAROS family zinc finger 2 (Helios)
Background Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.
Function Associates with Ikaros at centromeric heterochromatin. [UniProt]
Research Area Controls and Markers antibody; Gene Regulation antibody; Immune System antibody
Calculated MW 60 kDa