ARG62510
anti-Heparan Sulfate Proteoglycan 2 / Perlecan antibody [A7L6]
anti-Heparan Sulfate Proteoglycan 2 / Perlecan antibody [A7L6] for ICC/IF,IHC-Frozen sections and Human,Mouse,Pig
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rat Monoclonal antibody [A7L6] recognizes Heparan Sulfate Proteoglycan 2 / Perlecan |
|---|---|
| Tested Reactivity | Hu, Ms, Pig |
| Tested Application | ICC/IF, IHC-Fr |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | A7L6 |
| Isotype | IgG2a, kappa |
| Target Name | Heparan Sulfate Proteoglycan 2 / Perlecan |
| Antigen Species | Mouse |
| Immunogen | Murine EHS laminin preparation. |
| Conjugation | Un-conjugated |
| Alternate Names | PRCAN; Perlecan; SJS; SJS1; HSPG; PLC; SJA; Basement membrane-specific heparan sulfate proteoglycan core protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Tonsil |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified Antibody |
| Buffer | 1X PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P98160 Human Basement membrane-specific heparan sulfate proteoglycan core protein Swiss-port # Q05793 Mouse Basement membrane-specific heparan sulfate proteoglycan core protein |
|---|---|
| Gene Symbol | Hspg2 |
| Gene Full Name | perlecan (heparan sulfate proteoglycan 2) |
| Background | This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Function | Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development (By similarity). Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6 (By similarity). The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 469 kDa |
| PTM | Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. Perlecan contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation. |
Clone References
CXCR3-independent actions of the CXC chemokine CXCL10 in the infarcted myocardium and in isolated cardiac fibroblasts are mediated through proteoglycans.
A splice mutation and mRNA decay of EXT2 provoke hereditary multiple exostoses.
WB / Human
Distinct spatio-temporal extracellular matrix accumulation within demyelinated spinal cord lesions in Theiler's murine encephalomyelitis.
IHC-Fr / Mouse
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
IHC / Bovine
Heparan sulfate proteoglycans as regulators of fibroblast growth factor-2 signaling in brain endothelial cells. Specific role for glypican-1 in glioma angiogenesis.
WB / Mouse
