ARG54443

anti-Hsc 70 antibody [1B5]

anti-Hsc 70 antibody [1B5] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Bovine,Chicken,Dog,Guinea pig,Hamster,Human,Monkey,Mouse,Pig,Rabbit,Rat,Sheep

Signaling Transduction antibody

Overview

Product Description Rat Monoclonal antibody [1B5] recognizes Hsc 70
Tested Reactivity Hu, Ms, Rat, Bov, Chk, Dog, Gpig, Hm, Mk, Pig, Rb, Sheep
Tested Application ICC/IF, IHC-P, IP, WB
Specificity This antibody detects a 73 kDa protein on HeLa Heat Shocked Cell Lysate SDS-PAGE immunoblots, corresponding to the molecular weight of Hsc70. This antibody reacts with human, monkey, mouse, hamster, guinea pig, rat, rabbit, bovine, shep, pig, dog, and chicken Hsc70.
Host Rat
Clonality Monoclonal
Clone 1B5
Isotype IgG2a
Target Name Hsc 70
Antigen Species Hamster
Immunogen Hsc70 purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells.
Conjugation Un-conjugated
Alternate Names NIP71; HEL-33; HSPA10; LAP-1; HSC70; HSC71; LAP1; Lipopolysaccharide-associated protein 1; Heat shock 70 kDa protein 8; HSC54; HEL-S-72p; Heat shock cognate 71 kDa protein; HSP71; HSP73; LPS-associated protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 µg/ml
IHC-P5 µg/ml
IP5 µg/ml
WB0.1 -1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G Purified
Buffer PBS (pH 7.2) and 0.1mM PMSF in 50% Glycerol
Stabilizer 0.1mM PMSF in 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol Hspa8
Gene Full Name heat shock 70kDa protein 8
Background This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex (By similarity). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP (By similarity). [UniProt]
Research Area Signaling Transduction antibody
Calculated MW 71 kDa
PTM Acetylated.
ISGylated.
Trimethylation at Lys-561 reduces fibrillar SNCA binding.

Clone References

A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.

IP / 

Walczak CP et al.
PLoS Pathog.,  (2014)

publication_link

 

hr_line

MiR-17-5p impairs trafficking of H-ERG K+ channel protein by targeting multiple er stress-related chaperones during chronic oxidative stress.

WB / Rat, Human

Wang Q et al.
PLoS One.,  (2013)

publication_link

 

hr_line

scyllo-Inositol promotes robust mutant Huntingtin protein degradation.

Lai AY et al.
J Biol Chem.,  (2014)

publication_link

 

hr_line

Mutation in integrin-linked kinase (ILK(R211A)) and heat-shock protein 70 comprise a broadly cardioprotective complex.

WB / Human

Traister A et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Proteasome inhibition alleviates SNARE-dependent neurodegeneration.

WB / Mouse

Sharma M et al.
Sci Transl Med.,  (2012)

publication_link

 

hr_line

Overexpression of ST6GalNAcV, a ganglioside-specific alpha2,6-sialyltransferase, inhibits glioma growth in vivo.

IP / Human

Kroes RA et al.
Proc Natl Acad Sci U S A.,  (2010)

publication_link

 

hr_line

Photoreceptor IFT complexes containing chaperones, guanylyl cyclase 1 and rhodopsin.

IP / Cow

Bhowmick R et al.
Traffic.,  (2009)

publication_link

 

hr_line

Phosphorylation-dependent interaction of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHA) with PADI6 following oocyte maturation in mice.

IP / Human

Snow AJ et al.
Biol Reprod.,  (2008)

publication_link

 

hr_line