ARG62506

anti-Hsp 27 antibody [B317]

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0.5 ml

355.00

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Overview

Product Description	Mouse Monoclonal antibody [B317] recognizes Hsp 27
Tested Reactivity	Hu
Tested Application	IHC-Fr, IHC-P, WB
Host	Mouse
Clonality	Monoclonal
Clone	B317
Isotype	IgG1, kappa
Target Name	Hsp 27
Antigen Species	Human
Immunogen	Partially purified HSP27 derived from MCF-7 cytosol.
Conjugation	Un-conjugated
Alternate Names	HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28

Application Instructions

Application Suggestion

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control

Breast Carcinoma

Properties

Form	Liquid
Purification	Purified Antibody
Buffer	1X PBS and 0.1% Sodium azide
Preservative	0.1% Sodium azide
Concentration	0.2 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 3315 Human HSPB1 Swiss-port # P04792 Human Heat shock protein beta-1
Gene Symbol	HSPB1
Gene Full Name	heat shock 27kDa protein 1
Background	The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Function	Involved in stress resistance and actin organization. [UniProt]
Research Area	Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW	23 kDa
PTM	Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925).