ARG55588

anti-Hsp 40 antibody

anti-Hsp 40 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Hsp 40
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Hsp 40
Antigen Species Human
Immunogen Recombinant protein encoding full length of Human HSP40.
Conjugation Un-conjugated
Alternate Names Hsp40; HSP40; DnaJ protein homolog 1; hDj-1; HSPF1; Human DnaJ protein 1; Hdj1; RSPH16B; Heat shock 40 kDa protein 1; DnaJ homolog subfamily B member 1; Heat shock protein 40; Sis1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3337 Human DNAJB1

GeneID: 81489 Mouse DNAJB1

Swiss-port # P25685 Human DnaJ homolog subfamily B member 1

Swiss-port # Q9QYJ3 Mouse DnaJ homolog subfamily B member 1

Gene Symbol DNAJB1
Gene Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Background This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock
Research Area Signaling Transduction antibody
Calculated MW 38 kDa