ARG55956

anti-Hsp 60 antibody [LK1]

anti-Hsp 60 antibody [LK1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
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Overview

Product Description Mouse Monoclonal antibody [LK1] recognizes Hsp 60
Tested Reactivity Hu, Ms, Rat
Species Does Not React With Bacteria
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone LK1
Isotype IgG1, kappa
Target Name Hsp 60
Antigen Species Human
Immunogen Recombinant Human Hsp60 protein.
Conjugation Un-conjugated
Alternate Names Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF2 - 5 µg/ml
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15510 Mouse HSPD1

GeneID: 3329 Human HSPD1

GeneID: 63868 Rat HSPD1

Gene Symbol HSPD1
Gene Full Name heat shock 60kDa protein 1 (chaperonin)
Background This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
Function Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
Cellular Localization Mitochondria in cytoplasm
Research Area Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
Calculated MW 61 kDa

Customer's Feedback

nuts_pic      Excellent

Review for anti-Hsp 60 antibody [LK1]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-Hsp 60 antibody [LK1]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-Hsp 60 antibody [LK1]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Good

Review for anti-Hsp 60 antibody [LK1]

Application:WB

Sample:293T, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:0.4 µg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC