ARG23687
anti-HtrA1 antibody
anti-HtrA1 antibody for Western blot,ELISA and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes HtrA1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | The antibody reacts against the recombinant HtrA1 full length and truncated form of human HtrA1 prepared under oxidative conditions without DTT or under reducing conditions with DTT. Low cross reactivity of the antibody with the recombinant full length human HtrA3 and no cross reactivity with the recombinant full length human HtrA4 could be detected. The apparent MW of hHtrA1 in SDS-PAGE (in reducing conditions) is ~ 55 kDa. The truncated form appears in SDS-PAGE as a double band, with an apparent MW of ~ 40 kDa under reducing conditions and ~ 80 kDa under oxidative conditions (corresponds to the dimmer form of the truncated form of HtrA1). |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | HtrA1 |
| Antigen Species | Human |
| Immunogen | Human HtrA1 (full length). |
| Conjugation | Un-conjugated |
| Alternate Names | CARASIL; L56; HtrA; ARMD7; High-temperature requirement A serine peptidase 1; PRSS11; ORF480; EC 3.4.21.-; Serine protease 11; Serine protease HTRA1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. The IgG2a fraction was purified by affinity chromatography. |
| Buffer | PBS (pH 7.5), 20 mM Na-phosphate, 150 mM NaCl and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HTRA1 |
| Gene Full Name | HtrA serine peptidase 1 |
| Background | This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008] |
| Function | Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. [UniProt] |
| Calculated MW | 51 kDa |
