ARG57911

anti-HtrA2 / Omi antibody

anti-HtrA2 / Omi antibody for Immunoprecipitation,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody recognizes HtrA2 / Omi
Tested Reactivity Hu
Tested Application IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name HtrA2 / Omi
Antigen Species Human
Immunogen Recombinant Human HtrA2 / Omi protein.
Conjugation Un-conjugated
Alternate Names Serine protease HTRA2, mitochondrial; Serine proteinase OMI; Omi stress-regulated endoprotease; OMI; EC 3.4.21.108; PARK13; High temperature requirement protein A2; PRSS25; Serine protease 25; HtrA2

Application Instructions

Application Suggestion
Tested Application Dilution
IPAssay-dependent
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T
Observed Size ~ 36 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.03% Proclin300 and 50% Glycerol.
Preservative 0.03% Proclin300
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27429 Human HTRA2

Swiss-port # O43464 Human Serine protease HTRA2, mitochondrial

Gene Symbol HTRA2
Gene Full Name HtrA serine peptidase 2
Background This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq, Jul 2008]
Function Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. [UniProt]
Cellular Localization Mitochondrion intermembrane space_x000D_Mitochondrion membrane; Single-pass membrane protein. [UniProt]
Calculated MW 49 kDa
PTM Autoproteolytically activated. [UniProt]