ARG23689
anti-HtrA4 antibody
anti-HtrA4 antibody for Western blot,ELISA and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes HtrA4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | The antibody reacts against the recombinant HtrA4 full length prepared under oxidative conditions without DTT (see Figure –Panel A) or under reducing conditions with DTT. No cross reactivity of the antibody with the recombinant full length human HtrA1 or with the recombinant full length human HtrA3 could be detected. The recombinant HtrA4 appears in SDS-PAGE as a double band, with an apparent MW of ~47 kDa and respectively 12 kDa and in Western-blot with a main band at ~47 kDa. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | HtrA4 |
| Antigen Species | Human |
| Immunogen | Human HtrA4 (full length). |
| Conjugation | Un-conjugated |
| Alternate Names | Serine protease HTRA4; High-temperature requirement factor A4; EC 3.4.21.- |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS (pH 7.5), 20 mM Na-phosphate, 150 mM NaCl and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HTRA4 |
| Gene Full Name | HtrA serine peptidase 4 |
| Background | This gene encodes a member of the HtrA family of proteases. The encoded protein contains a putative signal peptide, an insulin growth factor binding domain, a Kazal protease inhibitor domain, a conserved trypsin domain and a PDZ domain. Based on studies on other related family members, this enzyme may function as a secreted oligomeric chaperone protease to degrade misfolded secretory proteins. Other human HtrA proteins have been implicated in arthritis, tumor suppression, unfolded stress response, apoptosis, and aging. [provided by RefSeq, Oct 2008] |
| Function | Serine protease. [UniProt] |
| Calculated MW | 51 kDa |
