ARG53948

anti-Human IgE antibody [BE5] (PE)

anti-Human IgE antibody [BE5] (PE) for Flow cytometry and Human

Immune System antibody

Overview

Product Description

PE-conjugated Mouse Monoclonal antibody [BE5] recognizes Human IgE

Tested Reactivity Hu
Tested Application FACS
Specificity The clone BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.
Host Mouse
Clonality Monoclonal
Clone BE5
Isotype IgG1
Target Name Human IgE
Antigen Species Human
Immunogen Purified human IgE.
Conjugation PE

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3497 Human IGHE

Background Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Research Area Immune System antibody

Clone References

Pharmacological characterization of MK-7246, a potent and selective CRTH2 (chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells) antagonist.

FACS / Human

Gervais FG et al.
Mol Pharmacol.,  (2011)

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