ARG44436
anti-IARS / Isoleucyl tRNA synthetase antibody
anti-IARS / Isoleucyl tRNA synthetase antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IARS / Isoleucyl tRNA synthetase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IARS / Isoleucyl tRNA synthetase |
| Antigen Species | Human |
| Immunogen | Human IARS / Isoleucyl tRNA synthetase recombinant protein |
| Conjugation | Un-conjugated |
| Alternate Names | IARS1; Isoleucyl-TRNA Synthetase 1; ILRS; IARS; Isoleucine TRNA Ligase 1, Cytoplasmic; Isoleucine--TRNA Ligase, Cytoplasmic; Isoleucyl-TRNA Synthetase |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified with Immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P41252 Human Isoleucine--tRNA ligase, cytoplasmic |
|---|---|
| Gene Symbol | IARS1 |
| Gene Full Name | Isoleucyl-TRNA Synthetase1 |
| Background | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. |
| Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. |
| Cellular Localization | Cytoplasm |
| Calculated MW | 144 kDa |
| PTM | Acetylation, Phosphoprotein |
