ARG54414
anti-ICAD antibody
anti-ICAD antibody for Western blot,Immunohistochemistry and Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes ICAD |
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Tested Reactivity | Ms |
Tested Application | IHC, WB |
Specificity | This antibody recognizes non-cleaved (45kDa) and cleaved mouse ICAD. |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | ICAD |
Antigen Species | Mouse |
Immunogen | Peptide corresponding to aa 2-21 at the N-terminus of mouse ICAD (accession no. O54786). |
Conjugation | Un-conjugated |
Alternate Names | DFF-45; DNA fragmentation factor 45 kDa subunit; Inhibitor of CAD; ICAD; DFF1; DNA fragmentation factor subunit alpha |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | Mouse lung |
Properties
Form | Liquid |
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Purification | Immunoaffinity chroma-tography |
Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
Preservative | 0.02% Sodium azide |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # O54786 Mouse DNA fragmentation factor subunit alpha |
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Gene Symbol | Dffa |
Gene Full Name | DNA fragmentation factor, alpha subunit |
Background | A human DNA fragmentation factor (DFF) that is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD (inhibitor of caspase-activated DNase). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Therefore, cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death. |
Function | Inhibitor of the caspase-activated DNase (DFF40). [UniProt] |
Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody |
Calculated MW | 37 kDa |
PTM | Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. |