ARG54414

anti-ICAD antibody

anti-ICAD antibody for Western blot,Immunohistochemistry and Mouse

Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ICAD
Tested Reactivity Ms
Tested Application IHC, WB
Specificity This antibody recognizes non-cleaved (45kDa) and cleaved mouse ICAD.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ICAD
Antigen Species Mouse
Immunogen Peptide corresponding to aa 2-21 at the N-terminus of mouse ICAD (accession no. O54786).
Conjugation Un-conjugated
Alternate Names DFF-45; DNA fragmentation factor 45 kDa subunit; Inhibitor of CAD; ICAD; DFF1; DNA fragmentation factor subunit alpha

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse lung

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13347 Mouse DFFA

Swiss-port # O54786 Mouse DNA fragmentation factor subunit alpha

Gene Symbol Dffa
Gene Full Name DNA fragmentation factor, alpha subunit
Background A human DNA fragmentation factor (DFF) that is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD (inhibitor of caspase-activated DNase). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Therefore, cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death.
Function Inhibitor of the caspase-activated DNase (DFF40). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 37 kDa
PTM Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.