ARG58092

anti-ID3 antibody

anti-ID3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ID3
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ID3
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-119 of Human ID3 (NP_002158.3).
Conjugation Un-conjugated
Alternate Names DNA-binding protein inhibitor ID-3; HEIR-1; Class B basic helix-loop-helix protein 25; ID-like protein inhibitor HLH 1R21; Inhibitor of DNA binding 3; Helix-loop-helix protein HEIR-1; bHLHb25; Inhibitor of differentiation 3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15903 Mouse ID3

GeneID: 25585 Rat ID3

GeneID: 3399 Human ID3

Gene Symbol ID3
Gene Full Name inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
Background The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the encoded protein lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it interacts. [provided by RefSeq, Aug 2011]
Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 13 kDa