ARG55897
anti-IDH2 antibody
anti-IDH2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,ICC/IF and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IDH2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IDH2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (17 aa) within the first 50 aa of Human IDH2. |
| Conjugation | Un-conjugated |
| Alternate Names | D2HGA2; IDH; Isocitrate dehydrogenase [NADP], mitochondrial; IDPM; EC 1.1.1.42; mNADP-IDH; ICD-M; IDP; IDHM; NADP; Oxalosuccinate decarboxylase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human heart tissue lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P48735 Human Isocitrate dehydrogenase [NADP], mitochondrial Swiss-port # P54071 Mouse Isocitrate dehydrogenase [NADP], mitochondrial |
|---|---|
| Gene Symbol | IDH2 |
| Gene Full Name | isocitrate dehydrogenase 2 (NADP+), mitochondrial |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| Function | Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. [UniProt] |
| Calculated MW | 51 kDa |
| PTM | Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. |
