ARG65798

anti-IDH2 antibody

anti-IDH2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig

Overview

Product Description Goat Polyclonal antibody recognizes IDH2
Tested Reactivity Hu, Ms, Rat, Pig
Predict Reactivity Cow, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IDH2
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human IDH2. (CIHGLSNVKLNE)
Conjugation Un-conjugated
Alternate Names D2HGA2; IDH; Isocitrate dehydrogenase [NADP], mitochondrial; IDPM; EC 1.1.1.42; mNADP-IDH; ICD-M; IDP; IDHM; NADP; Oxalosuccinate decarboxylase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 269951 Mouse IDH2

GeneID: 3418 Human IDH2

GeneID: 361596 Rat IDH2

Gene Symbol IDH2
Gene Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Background Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Function Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. [UniProt]
Calculated MW 51 kDa
PTM Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.