ARG55413

anti-IFIH1 / MDA5 antibody

anti-IFIH1 / MDA5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes IFIH1 / MDA5
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IFIH1 / MDA5
Antigen Species Human
Immunogen Synthetic peptide (16 aa) within the last 50 aa of Human MDA5.
Conjugation Un-conjugated
Alternate Names Interferon-induced helicase C domain-containing protein 1; Murabutide down-regulated protein; SGMRT1; EC 3.6.4.13; RIG-I-like receptor 2; MDA5; Clinically amyopathic dermatomyositis autoantigen 140 kDa; AGS7; MDA-5; CADM-140 autoantigen; Melanoma differentiation-associated protein 5; IDDM19; Interferon-induced with helicase C domain protein 1; RNA helicase-DEAD box protein 116; Helicard; RLR-2; Hlcd; Helicase with 2 CARD domains

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF20 μg/ml
IHC-P5 μg/ml
WB1 - 4 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Daudi Cell Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64135 Human IFIH1

GeneID: 71586 Mouse IFIH1

Swiss-port # Q8R5F7 Mouse Interferon-induced helicase C domain-containing protein 1

Swiss-port # Q9BYX4 Human Interferon-induced helicase C domain-containing protein 1

Gene Symbol IFIH1
Gene Full Name interferon induced with helicase C domain 1
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]
Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. [UniProt]
Highlight Related products:
IFIH1 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Research Area Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 117 kDa
PTM Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta.
Ubiquitinated by RNF125, leading to its degradation by the proteasome (PubMed:17460044). USP17/UPS17L2-dependent deubiquitination positively regulates the receptor (PubMed:20368735).
During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.