ARG63323

anti-IFIH1 / MDA5 antibody

anti-IFIH1 / MDA5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Monkey

Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description Goat Polyclonal antibody recognizes IFIH1 / MDA5
Tested Reactivity Hu, Mk
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IFIH1 / MDA5
Antigen Species Human
Immunogen SNGYSTDENFRYL-C
Conjugation Un-conjugated
Alternate Names Interferon-induced helicase C domain-containing protein 1; Murabutide down-regulated protein; SGMRT1; EC 3.6.4.13; RIG-I-like receptor 2; MDA5; Clinically amyopathic dermatomyositis autoantigen 140 kDa; AGS7; MDA-5; CADM-140 autoantigen; Melanoma differentiation-associated protein 5; IDDM19; Interferon-induced with helicase C domain protein 1; RNA helicase-DEAD box protein 116; Helicard; RLR-2; Hlcd; Helicase with 2 CARD domains

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P10 µg/ml
WBAssay - dependent
Application Note IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64135 Human IFIH1

Swiss-port # Q9BYX4 Human Interferon-induced helicase C domain-containing protein 1

Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]
Highlight Related products:
IFIH1 antibodies; Anti-Goat IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Research Area Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 117 kDa
PTM Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta.
Ubiquitinated by RNF125, leading to its degradation by the proteasome (PubMed:17460044). USP17/UPS17L2-dependent deubiquitination positively regulates the receptor (PubMed:20368735).
During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.