ARG23186
anti-IFN gamma antibody [7B6]
anti-IFN gamma antibody [7B6] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Bovine,Sheep,Deer,Goat
Overview
| Product Description | Mouse Monoclonal antibody [7B6] recognizes IFN gamma |
|---|---|
| Tested Reactivity | Bov, Deer, Goat, Sheep |
| Tested Application | FACS, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7B6 |
| Isotype | IgG1 |
| Target Name | IFN gamma |
| Antigen Species | Bovine |
| Conjugation | Un-conjugated |
| Alternate Names | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Membrane permeabilization is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IFNG |
|---|---|
| Gene Full Name | interferon, gamma |
| Background | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015] |
| Function | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| Highlight | Related products: IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| Calculated MW | 19 kDa |
| PTM | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. [UniProt] |
