ARG21429

anti-IFN gamma antibody [B27] (Biotin)

anti-IFN gamma antibody [B27] (Biotin) for ELISA,ELISPOT,Flow cytometry,ICC/IF,IHC-Frozen sections,Neutralizing and African green monkey,Chimpanzee,Human,Macaque,Rhesus Monkey

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [B27 ] recognizes IFN gamma
Tested Reactivity Hu, AGMK, Chimp, Macaq, R. Mk
Tested Application ELISA, ELISPOT, FACS, ICC/IF, IHC-Fr, Neut
Specificity Human/Rhesus/Cynomolgus/Chimpanzee/Pigtail Macaque/African Green Monkey/Sooty Mangabey IFN-γ.
Host Mouse
Clonality Monoclonal
Clone B27
Isotype IgG1, kappa
Target Name IFN gamma
Antigen Species Human
Immunogen E. coli-expressed human IFN-γ
Conjugation Biotin
Alternate Names IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000 - 1:8000
ELISPOTAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
NeutAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3458 Human IFNG

Swiss-port # P01579 Human Interferon gamma

Gene Symbol IFNG
Gene Full Name interferon, gamma
Background This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
Highlight Related products:
IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Anti-Mouse IgG secondary antibodies;
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Inflammatory Cytokines
Calculated MW 19 kDa
PTM Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.