ARG63880

anti-IFT88 / Polaris antibody

anti-IFT88 / Polaris antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes IFT88 / Polaris
Tested Reactivity Hu, Ms
Predict Reactivity Dog, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2)
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IFT88 / Polaris
Antigen Species Human
Immunogen C-KKRIDEDDFADEE
Conjugation Un-conjugated
Alternate Names Recessive polycystic kidney disease protein Tg737 homolog; hTg737; D13S1056E; Intraflagellar transport protein 88 homolog; TG737; TPR repeat protein 10; TTC10; Tetratricopeptide repeat protein 10; DAF19

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P3 µg/ml
WB0.3 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21821 Mouse IFT88

GeneID: 8100 Human IFT88

Swiss-port # Q13099 Human Intraflagellar transport protein 88 homolog

Swiss-port # Q61371 Mouse Intraflagellar transport protein 88 homolog

Background This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
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Research Area Gene Regulation antibody
Calculated MW 94 kDa