ARG54644

anti-IGF1 antibody [0.T.61]

anti-IGF1 antibody [0.T.61] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Neutralizing,Western blot and Mouse,Rat

Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [0.T.61] recognizes IGF1

Tested Reactivity Ms, Rat
Predict Reactivity Chk
Tested Application IHC-P, IP, Neut, WB
Specificity Recognizes human Insulin-Like Growth Factor I (IGF1), Mr ~17kD (migrates to ~7kD). Crossreactivity with IGF-II is ~40%.
Host Mouse
Clonality Monoclonal
Clone 0.T.61
Isotype IgG1, kappa
Target Name IGF1
Antigen Species Human
Immunogen Human insulin-like growth factor I (IGF1) purified from human plasma.
Conjugation Un-conjugated
Alternate Names MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P10 µg/ml
IP5 µg for 100 ng IGF1
Neut10 - 20 µg/ml
WB0.5-2 µg/ml of antibody detects 100 ng of IGF1 under non-reducing conditions.
Application Note Neut: 10-20 µg/ml can inhibit the activity of 10 ng/ml of IGF1 as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: formalin-fixed, paraffin-embedded skin sections.

Properties

Form Liquid
Purification Affinity purification with Protein G.
Buffer 0.1M Tris-glycine (pH 7.4), 0.15 mM sodium chloride and 0.1mM EDTA
Stabilizer 0.1mM EDTA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16000 Mouse IGF1

GeneID: 24482 Rat IGF1

Swiss-port # P05017 Mouse Insulin-like growth factor I

Swiss-port # P08025 Rat Insulin-like growth factor I

Gene Symbol IGF1
Gene Full Name insulin-like growth factor 1 (somatomedin C)
Background The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. [UniProt]
Research Area Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 22 kDa