ARG56665

anti-IGF1 antibody

anti-IGF1 antibody for ELISA,Western blot,Neutralizing and Mouse

Overview

Product Description Goat Polyclonal antibody recognizes IGF1
Tested Reactivity Ms
Tested Application ELISA, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IGF1
Antigen Species Mouse
Immunogen E.coli derived Recombinant Mouse IGF1.
(GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA)
Conjugation Un-conjugated
Alternate Names MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56775 as a detection antibody
Neut12.0 - 14.0 µg/ml (To yield [ND50] of the biological activity of Murine IGF - I (200 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16000 Mouse IGF1

Swiss-port # P05017 Mouse Insulin-like growth factor I

Gene Symbol Igf1
Gene Full Name insulin-like growth factor 1
Background The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. [UniProt]
Calculated MW 22 kDa