ARG65599

anti-IGF1 antibody

anti-IGF1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description

Goat Polyclonal antibody recognizes IGF1

Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognize all reported isoforms (NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IGF1
Antigen Species Human
Immunogen C-RSVRAQRHTD
Conjugation Un-conjugated
Alternate Names MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P3 - 5 µg/ml
WB0.5 - 2 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Uterus lysate

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3479 Human IGF1

Swiss-port # P05019 Human Insulin-like growth factor I

Background The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
Research Area Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 22 kDa