ARG66585

anti-IGFBP3 antibody

anti-IGFBP3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes IGFBP3
Tested Reactivity Hu, Ms
Predict Reactivity Bov, Pig
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IGFBP3
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide within the center region of Human IGFBP3.
Conjugation Un-conjugated
Alternate Names IBP-3; IBP3; Insulin-like growth factor-binding protein 3; IGFBP-3; IGF-binding protein 3; BP-53

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 42 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16009 Mouse IGFBP3

GeneID: 3486 Human IGFBP3

Swiss-port # P17936 Human Insulin-like growth factor-binding protein 3

Swiss-port # P47878 Mouse Insulin-like growth factor-binding protein 3

Gene Symbol IGFBP3
Gene Full Name insulin-like growth factor binding protein 3
Background This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 32 kDa
PTM Phosphorylated by FAM20C in the extracellular medium. [UniProt]