ARG54650
anti-IKK alpha antibody
anti-IKK alpha antibody for ELISA,ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IKK alpha |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, ICC/IF, WB |
| Specificity | Antibody has no cross response to IKKb or IKKg. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IKK alpha |
| Immunogen | Synthetic peptide (19 aa) within the last 50 aa of Human IKK alpha. |
| Conjugation | Un-conjugated |
| Alternate Names | Conserved helix-loop-helix ubiquitous kinase; IKK-A; TCF16; IKK1; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase 1; IkappaB kinase; Transcription factor 16; EC 2.7.11.10; Nuclear factor NF-kappa-B inhibitor kinase alpha; IkBKA; IKBKA; IKK-alpha; NFKBIKA; IKKA; TCF-16; I-kappa-B kinase alpha |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa Cell Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15111 Human Inhibitor of nuclear factor kappa-B kinase subunit alpha |
|---|---|
| Gene Symbol | CHUK |
| Gene Full Name | conserved helix-loop-helix ubiquitous kinase |
| Background | IKK alpha Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IkB-alpha. IKKα is expressed in variety of human tissues. | |
| Highlight | Related Antibody Duos and Panels: ARG30255 IKB alpha degradation Antibody Panel (IKK alpha, IKK beta, IKB alpha) Related products: IKK alpha antibodies; IKK alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related poster download: Toll-like Receptor.pdf |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody |
| Calculated MW | 85 kDa |
| PTM | Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated. (Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway. |
