ARG56668

anti-IL1 alpha antibody

anti-IL1 alpha antibody for ELISA,Western blot,Neutralizing,IHC-Frozen sections and Rat

Overview

Product Description Goat Polyclonal antibody recognizes IL1 alpha
Tested Reactivity Rat
Tested Application ELISA, IHC-Fr, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IL1 alpha
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat IL-1α.
(MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS)
Conjugation Un-conjugated
Alternate Names IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56778 as a detection antibody
IHC-Fr1.0 µg/ml
Neut0.011 - 0.017 µg/ml (To yield [ND50] of the biological activity of Rat IL - 1α (50 pg/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24493 Rat IL1A

Swiss-port # P16598 Rat Interleukin-1 alpha

Gene Symbol Il1a
Gene Full Name interleukin 1 alpha
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
Highlight Related products:
IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins; Anti-Goat IgG secondary antibodies;
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Calculated MW 31 kDa