ARG22429

anti-IL1 beta antibody [1D4]

anti-IL1 beta antibody [1D4] for IHC-Frozen sections,ELISA,Flow cytometry,Western blot and Sheep,Goat,Bovine,Horse

Pyroptosis Study antibody

Overview

Product Description Mouse Monoclonal antibody [1D4] recognizes IL1 beta
This antibody recognizes ovine interleukin-1 beta, and shows no cross-reactivity with ovine IL-6, IL-8, TNF alpha or MCP-1.Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 demonstrates partial neutralizing activity of ovine IL-1 beta.
Tested Reactivity Bov, Goat, Hrs, Sheep
Tested Application ELISA, FACS, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 1D4
Isotype IgG1
Target Name IL1 beta
Antigen Species Sheep
Immunogen Recombinant ovine IL-1 Beta
Conjugation Un-conjugated
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1:10
IHC-FrAssay-dependent
WBAssay-dependent
Application Note FACS: Membrane permeabilisation is required for this application. Arigo recommends the use of Leucoperm for this purpose. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 281251 Bovine IL1B

Swiss-port # P09428 Bovine Interleukin-1 beta

Gene Symbol IL1B
Gene Full Name interleukin 1 beta
Background IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
Highlight Related products:
IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins; Anti-Mouse IgG secondary antibodies;
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Research Area Pyroptosis Study antibody
Calculated MW 31 kDa
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.