ARG22451

anti-IL10 antibody [CC318]

anti-IL10 antibody [CC318] for ELISA,ELISPOT,Flow cytometry and Horse,Sheep,Bovine

Overview

Product Description Mouse Monoclonal antibody [CC318] recognizes IL10
This antibody recognizes bovine IL-10. Mouse anti Bovine Interleukin-10 antibody, clone CC318 has been shown not to inhibit the biological activity of IL-10.
Tested Reactivity Bov, Hrs, Sheep
Tested Application ELISA, ELISPOT, FACS
Host Mouse
Clonality Monoclonal
Clone CC318
Isotype IgG2b
Target Name IL10
Antigen Species Bovine
Immunogen Plasmid cDNA encoding bovine IL-10.
Conjugation Un-conjugated
Alternate Names IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA5 - 10 µg/ml
ELISPOTAssay-dependent
FACSAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 281246 Bovine IL10

Swiss-port # P43480 Bovine Interleukin-10

Gene Symbol IL10
Gene Full Name interleukin 10
Background The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt]
Calculated MW 21 kDa