ARG21422
anti-IL10 antibody [JES3-12G8] (Biotin)
anti-IL10 antibody [JES3-12G8] (Biotin) for ELISA,ELISPOT,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Neutralizing,Western blot and Human,Virus
Overview
| Product Description | Biotin-conjugated Rat Monoclonal antibody [JES3-12G8 ] recognizes IL10 |
|---|---|
| Tested Reactivity | Hu, Virus |
| Tested Application | ELISA, ELISPOT, IHC-Fr, IHC-P, Neut, WB |
| Specificity | The IL10 antibody clone JES3-12G8 reacts with human interleukin-10 (IL-10) and viral IL-10. Might also react to Cynomolgus IL-10. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | JES3-12G8 |
| Isotype | IgG2a, kappa |
| Target Name | IL10 |
| Antigen Species | Human |
| Immunogen | COS-expressed human IL-10 |
| Conjugation | Biotin |
| Alternate Names | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
Application Instructions
| Application Suggestion |
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| Application Note | ELISA: The clone JES3-12G8 may be paired with the purified clone JES3-9D7 in a sandwich ELISA. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL10 |
| Gene Full Name | interleukin 10 |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
| Function | Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt] |
| Calculated MW | 21 kDa |
