ARG10202
anti-IL16 antibody [398]
anti-IL16 antibody [398] for ELISA and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [398] recognizes Human IL-16 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | mAb 398 exhibits no detectable cross-reaction with human serum albumin, bovine serum albumin and other human cytokines or growth factors tested, such as IL-1β, IL-8, IL-7 MCAF, TGF-β1, EGF, bFGF, GM-CSF, M-CSF, TNF-α. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 398 |
| Isotype | IgG1, kappa |
| Target Name | IL16 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human IL-16 |
| Conjugation | Un-conjugated |
| Alternate Names | prIL-16; Lymphocyte chemoattractant factor; PRIL16; IL-16; NIL16; LCF; Pro-interleukin-16 |
Application Instructions
| Application Note | ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human IL-16 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: ARG10203). Suggested Capture Coating Dose: 0.3μg/well; Substrate: TMB. If the above suggested conditions are followed, approximately 20pg/mL of IL-16 in serum/plasma or 15 pg/mL of IL-16 in cell medium/urine can be detected with an assay range of 0-1000 pg/mL. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.2) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL16 |
| Gene Full Name | interleukin 16 |
| Background | Interleukin 16 (IL-16) is a pleiotropic cytokine, possessing immunomodulation function to allergic inflammatory diseases such as Crohn's disease and ulcerative colitis. IL-16 utilizes CD4 as its primary receptor. IL-16 is a chemo-attractant to CD4+ T helper 1 cells. To CD4+ T helper 2 cells, IL-16 has an inhibitory effect on the antigen-mediated CD3/T cell receptor activation. IL16 has been shown to suppress HIV infection in lymphocytes and monocytes. |
| Function | Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane. Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30075 IL16 ELISA Antibody Duo Related products: IL16 antibodies; IL16 ELISA Kits; IL16 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 142 kDa |
| PTM | Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. |
