ARG41569

anti-IL18 antibody

anti-IL18 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes IL18
Tested Reactivity Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL18
Antigen Species Mouse
Immunogen Recombinant protein corresponding to N36-S192 of Mouse IL18.
Conjugation Un-conjugated
Alternate Names Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 22 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 0.05% Sodium azide.
Preservative 0.05% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16173 Mouse IL18

Swiss-port # P70380 Mouse Interleukin-18

Gene Symbol IL18
Gene Full Name interleukin 18
Background The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 22 kDa
PTM The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt]