ARG23961
anti-IL21 antibody [SQab19145] (low endotoxin)
anti-IL21 antibody [SQab19145] (low endotoxin) for Neutralizing and Human,Rhesus Monkey,Macaque
Overview
| Product Description | Low endotoxin Mouse Monoclonal antibody [SQab19145] recognizes IL21 |
|---|---|
| Tested Reactivity | Hu, Macaq, R. Mk |
| Tested Application | Neut |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab19145 |
| Isotype | IgG1 |
| Target Name | IL21 |
| Antigen Species | Human |
| Immunogen | Recombinant Human IL21 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Za11; IL-21; CVID11; Interleukin-21 |
Application Instructions
| Application Suggestion |
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| Application Note | Neutralization dose 50 (ND50): The antibody neutralized the effect of IL21 at a ND50 value of ~ 0.05 µg/ml when HEK cells were activated with 10 ng/ml of IL21. The corresponding ND50 value for Human PBMC activated with 10 ng/ml IL21 was ~ 0.2 µg/ml. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL21 |
| Gene Full Name | interleukin 21 |
| Background | This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
| Function | Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 19 kDa |
