ARG62580
anti-INK4c antibody [18P118 (DCS-118)]
anti-INK4c antibody [18P118 (DCS-118)] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,ELISA and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [18P118 (anti DCS-118)] recognizes INK4c |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 18P118 (DCS-118) |
| Isotype | IgG1 |
| Target Name | INK4c |
| Immunogen | Recombinant full length protein |
| Conjugation | Un-conjugated |
| Alternate Names | p18-INK4C; Cyclin-dependent kinase 6 inhibitor; Cyclin-dependent kinase 4 inhibitor C; p18-INK6; p18-INK4c; p18; INK4C |
Application Instructions
| Application Note | WB: 1 - 2 µg/ml IP: 1-2 µg/mg of lysate IHC-P: 2 - 4 µg/ml ELISA: 5 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | HeLa cells. Tonsil |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P42773 Human Cyclin-dependent kinase 4 inhibitor C |
|---|---|
| Gene Symbol | CDKN2C |
| Gene Full Name | cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4) |
| Background | The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008] |
| Function | Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 18 kDa |
Clone References
An opposite effect of the CDK inhibitor, p18(INK4c) on embryonic stem cells compared with tumor and adult stem cells.
WB / Mouse
Upregulation of p18Ink4c expression by oncogenic HPV E6 via p53-miR-34a pathway.
WB / Human
The cyclin-dependent kinase inhibitors p15INK4B and p21CIP1 are critical regulators of fibrillar collagen-induced tumor cell cycle arrest.
WB / Human
Proliferation and differentiation in isogenic populations of peripheral B cells activated by Epstein-Barr virus or T cell-derived mitogens.
WB / Human
