ARG66734

anti-INTS5 antibody

anti-INTS5 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes INTS5
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name INTS5
Antigen Species Human
Immunogen Recombinant full length protein of Human INTS5.
Conjugation Un-conjugated
Alternate Names INT5; KIAA1698; Integrator complex subunit 5; Int5

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7, HT29 and Mouse liver
Observed Size ~ 120 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 109077 Mouse INTS5

GeneID: 80789 Human INTS5

Swiss-port # Q6P9B9 Human Integrator complex subunit 5

Swiss-port # Q8CHT3 Mouse Integrator complex subunit 5

Gene Symbol INTS5
Gene Full Name integrator complex subunit 5
Background The Integrator complex is a complex that associates with the C-terminal domain of RNA polymerase II large subunit. This complex is brought to U1 and U2 small nuclear RNA genes, where it is involved in the transcription and processing of their transcripts. The protein encoded by this gene represents a subunit of the Integrator complex. [provided by RefSeq, Aug 2016]
Function Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex (PubMed:23904267). [UniProt]
Cellular Localization Nucleus membrane; Multi-pass membrane protein. Nucleus. Cytoplasm. [UniProt]
Calculated MW 108 kDa