ARG54631
anti-IRAK4 antibody
anti-IRAK4 antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IRAK4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody specifically recognizeshuman IRAK-4 (IL-1 ReceptorAssociated Kinase-4). This antibodydoes not cross-react with other IRAKs. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IRAK4 |
| Antigen Species | Human |
| Immunogen | A synthetic peptidecorresponding to amino acids at thecarboxy terminus of human IRAK-4. |
| Conjugation | Un-conjugated |
| Alternate Names | REN64; Renal carcinoma antigen NY-REN-64; NY-REN-64; Interleukin-1 receptor-associated kinase 4; EC 2.7.11.1; IRAK-4; IPD1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa and K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | purified by Immunoaffinity chromatography. |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NWZ3 Human Interleukin-1 receptor-associated kinase 4 |
|---|---|
| Gene Symbol | IRAK4 |
| Gene Full Name | interleukin-1 receptor-associated kinase 4 |
| Background | IRAK-4 activates the NF-B and MAPKpathways and plays a role in IL-1Rmediated inflammatory responses andinnate immunity. IRAK-4-deficientanimals are resistant to challenge withLPS and are impaired in their responsesto viral and bacterial challenges. |
| Function | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. [UniProt] |
| Highlight | Related products: IRAK4 antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The NF-kappa B Pathways.pdf Toll-like Receptor.pdf |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 52 kDa |
| PTM | Phosphorylated. |
Customer's Feedback
Average
Review for anti-IRAK4 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
