ARG40603
anti-IRE1a phospho (Ser724) antibody
anti-IRE1a phospho (Ser724) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IRE1a phospho (Ser724) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IRE1a |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Ser724 of Human IRE1a. |
| Conjugation | Un-conjugated |
| Alternate Names | Ire1-alpha; Serine/threonine-protein kinase/endoribonuclease IRE1; IRE1a; Endoplasmic reticulum-to-nucleus signaling 1; EC 3.1.26.-; IRE1; Inositol-requiring protein 1; IRE1P; EC 2.7.11.1; hIRE1p |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa + Calyculin A | ||||||
| Observed Size | 130 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O75460 Human Serine/threonine-protein kinase/endoribonuclease IRE1 Swiss-port # Q9EQY0 Mouse Serine/threonine-protein kinase/endoribonuclease IRE1 |
|---|---|
| Gene Symbol | ERN1 |
| Gene Full Name | endoplasmic reticulum to nucleus signaling 1 |
| Background | The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008] |
| Function | Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 110 kDa |
| PTM | Autophosphorylated. ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities. [UniProt] |
