ARG40739

anti-IRE1a phospho (Ser724) antibody

anti-IRE1a phospho (Ser724) antibody for Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes IRE1a phospho (Ser724)
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IRE1a
Antigen Species Human
Immunogen Phosphospecific peptide around Ser724 of Human IRE1a.
Conjugation Un-conjugated
Alternate Names Ire1-alpha; Serine/threonine-protein kinase/endoribonuclease IRE1; IRE1a; Endoplasmic reticulum-to-nucleus signaling 1; EC 3.1.26.-; IRE1; Inositol-requiring protein 1; IRE1P; EC 2.7.11.1; hIRE1p

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2081 Human ERN1

Swiss-port # O75460 Human Serine/threonine-protein kinase/endoribonuclease IRE1

Gene Symbol ERN1
Gene Full Name endoplasmic reticulum to nucleus signaling 1
Background The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]
Function Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 110 kDa
PTM Autophosphorylated.

ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities. [UniProt]