ARG63033
anti-IgE antibody [4G7]
anti-IgE antibody [4G7] for ELISA,Flow cytometry and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [4G7] recognizes IgE |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS |
| Specificity | The clone 4G7 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4G7 |
| Isotype | IgG |
| Target Name | IgE |
| Antigen Species | Human |
| Immunogen | Purified human IgE. |
| Epitope | Located within aa. 103-115 (WSDYNFDYSSSEE). |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by precipitation methods and ion exchange chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion. |
| Research Area | Immune System antibody |
Clone References
The molecular basis of antigenic cross-reactivity between the group 2 mite allergens.
Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2.
