ARG63034
anti-IgE antibody [4H10]
anti-IgE antibody [4H10] for ELISA,Flow cytometry and Human
Immune System antibody
Overview
Product Description | Mouse Monoclonal antibody [4H10] recognizes IgE |
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Tested Reactivity | Hu |
Tested Application | ELISA, FACS |
Specificity | The clone 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. |
Host | Mouse |
Clonality | Monoclonal |
Clone | 4H10 |
Isotype | IgG1 |
Target Name | IgE |
Antigen Species | Human |
Immunogen | Purified human IgE. |
Conjugation | Un-conjugated |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from ascites by precipitation methods and ion exchange chromatography. |
Purity | > 95% (by SDS-PAGE) |
Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
Preservative | 15 mM Sodium azide |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Background | Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion. |
Research Area | Immune System antibody |